Opposing effects of fibroblast growth factor and pertussis toxin on alkaline phosphatase, osteopontin, osteocalcin, and type I collagen mRNA levels in ROS 17/2.8 cells
SB Rodan, G Wesolowski, K Yoon… - Journal of Biological …, 1989 - ASBMB
In rat osteosarcoma (ROS 17/2.8) cells, which express osteoblastic features in culture, basic
fibroblast growth factor (bFGF) reduces the level of alkaline phosphatase, type I collagen,
and osteocalcin mRNA and increases osteopontin mRNA, independent of growth
stimulation. The fibroblast growth factor (FGF) effects are dose dependent (EC 50 about 6
pM) and are detected 24 h after addition of the growth factor. bFGF also reduces parathyroid
hormone-stimulatable adenylate cyclase and alkaline phosphatase activity in these cells …
fibroblast growth factor (bFGF) reduces the level of alkaline phosphatase, type I collagen,
and osteocalcin mRNA and increases osteopontin mRNA, independent of growth
stimulation. The fibroblast growth factor (FGF) effects are dose dependent (EC 50 about 6
pM) and are detected 24 h after addition of the growth factor. bFGF also reduces parathyroid
hormone-stimulatable adenylate cyclase and alkaline phosphatase activity in these cells …
