Expression and regulation of basic fibroblast growth factor mRNA levels in mouse osteoblastic MC3T3-E1 cells.
MM Hurley, C Abreu, G Gronowicz… - Journal of biological …, 1994 - ASBMB
Basic fibroblast growth factor (bFGF) is a potent mitogen for bone cells and is a constituent of
the bone matrix. We have found that osteoblastic MC3T3-E1 cells expressed bFGF mRNA
transcript of 4.5 kilobases (kb). We examined factors that regulate the expression of bFGF
mRNA and protein in MC3T3-E1 cells. Treatment of MC3T3-E1 cells with bFGF (10 nM) for 4-
48 h induced another 7-kb bFGF transcript at 4 h. Treatment of MC3T3-E1 cells with TGF
beta (10 ng/ml) also induced the 7-kb transcript of bFGF mRNA. In contrast, heparin …
the bone matrix. We have found that osteoblastic MC3T3-E1 cells expressed bFGF mRNA
transcript of 4.5 kilobases (kb). We examined factors that regulate the expression of bFGF
mRNA and protein in MC3T3-E1 cells. Treatment of MC3T3-E1 cells with bFGF (10 nM) for 4-
48 h induced another 7-kb bFGF transcript at 4 h. Treatment of MC3T3-E1 cells with TGF
beta (10 ng/ml) also induced the 7-kb transcript of bFGF mRNA. In contrast, heparin …
