[HTML][HTML] α-Thrombin induces rapid and sustained Akt phosphorylation by β-arrestin1-dependent and-independent mechanisms, and only the sustained Akt …
In Chinese hamster embryonic fibroblasts (IIC9 cells) α-thrombin activates the MAPK (ERK)
and phosphatidylinositol 3-OH-kinase (PI 3-kinase)/Akt pathways, and both are essential for
progression through the G 1 phase of the cell cycle. We investigated in IIC9 cells, the role of
β-arrestin1 in α-thrombin signaling to these pathways. α-Thrombin stimulates rapid and
sustained PI 3-kinase and Akt activities. Expression of a dominant negative β-arrestin1 (β-
arrestin1 (V53D)) inhibits rapid but not sustained PI 3-kinase and Akt activities. Surprisingly …
and phosphatidylinositol 3-OH-kinase (PI 3-kinase)/Akt pathways, and both are essential for
progression through the G 1 phase of the cell cycle. We investigated in IIC9 cells, the role of
β-arrestin1 in α-thrombin signaling to these pathways. α-Thrombin stimulates rapid and
sustained PI 3-kinase and Akt activities. Expression of a dominant negative β-arrestin1 (β-
arrestin1 (V53D)) inhibits rapid but not sustained PI 3-kinase and Akt activities. Surprisingly …