In vitro selection using modified or unnatural nucleotides
SM Knudsen, MP Robertson… - Current Protocols in …, 2001 - Wiley Online Library
SM Knudsen, MP Robertson, AD Ellington
Current Protocols in Nucleic Acid Chemistry, 2001•Wiley Online LibraryThe use of modified nucleotides in an RNA or DNA pool to be used for in vitro selection
offers many potential advantages, such as the increased stability of the selected nucleic acid
against nuclease degradation. This unit provides useful information and protocols for in vitro
selection using modified nucleotides. It includes a discussion of when to use modified
nucleotides; protocols for preparing a modified RNA pool and verifying its suitability for in
vitro selection; and protocols for selecting and amplifying a functionally enriched pool.
offers many potential advantages, such as the increased stability of the selected nucleic acid
against nuclease degradation. This unit provides useful information and protocols for in vitro
selection using modified nucleotides. It includes a discussion of when to use modified
nucleotides; protocols for preparing a modified RNA pool and verifying its suitability for in
vitro selection; and protocols for selecting and amplifying a functionally enriched pool.
Abstract
The use of modified nucleotides in an RNA or DNA pool to be used for in vitro selection offers many potential advantages, such as the increased stability of the selected nucleic acid against nuclease degradation. This unit provides useful information and protocols for in vitro selection using modified nucleotides. It includes a discussion of when to use modified nucleotides; protocols for preparing a modified RNA pool and verifying its suitability for in vitro selection; and protocols for selecting and amplifying a functionally enriched pool.
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