[HTML][HTML] Dynamin is functionally coupled to insulin granule exocytosis
The insulin granule integral membrane protein marker phogrin-green fluorescent protein
was co-localized with insulin in Min6B1 β-cell secretory granules but did not undergo
plasma membrane translocation following glucose stimulation. Surprisingly, although
expression of a dominant-interfering dynamin mutant (Dyn/K44A) inhibited transferrin
receptor endocytosis, it had no effect on phogringreen fluorescent protein localization in the
basal or secretagogue-stimulated state. By contrast, co-expression of Dyn/K44A with human …
was co-localized with insulin in Min6B1 β-cell secretory granules but did not undergo
plasma membrane translocation following glucose stimulation. Surprisingly, although
expression of a dominant-interfering dynamin mutant (Dyn/K44A) inhibited transferrin
receptor endocytosis, it had no effect on phogringreen fluorescent protein localization in the
basal or secretagogue-stimulated state. By contrast, co-expression of Dyn/K44A with human …