Crosslinking of ANCA-antigens stimulates superoxide release by human neutrophils.
R Kettritz, JC Jennette, RJ Falk - Journal of the American Society …, 1997 - journals.lww.com
Anti-neutrophil cytoplasmic antibodies (ANCA) activate primed human polymorphonuclear
neutrophils (PMN) in vitro, resulting in a respiratory burst and degranulation. In this study,
the hypotheses that the initiation of this process requires engagement of the F (ab') 2 portion
of ANCA, and that crosslinking of ANCA target antigens is necessary to trigger superoxide
(O2-) release, were explored. It is speculated that Fc gamma receptor engagement is a
modulator of ANCA-mediated activation. Flow cytometry demonstrated that intact human …
neutrophils (PMN) in vitro, resulting in a respiratory burst and degranulation. In this study,
the hypotheses that the initiation of this process requires engagement of the F (ab') 2 portion
of ANCA, and that crosslinking of ANCA target antigens is necessary to trigger superoxide
(O2-) release, were explored. It is speculated that Fc gamma receptor engagement is a
modulator of ANCA-mediated activation. Flow cytometry demonstrated that intact human …
