Characterization of a selective inhibitor of inositol hexakisphosphate kinases
Inositol hexakisphosphate kinases (IP6Ks) phosphorylate inositol hexakisphosphate (InsP
6) to yield 5-diphosphoinositol pentakisphosphate (5-[PP]-InsP 5 or InsP 7). In this study, we
report the characterization of a selective inhibitor, N 2-(m-(trifluoromethy) lbenzyl) N 6-(p-
nitrobenzyl) purine (TNP), for these enzymes. TNP dose-dependently and selectively
inhibited the activity of IP6K in vitro and inhibited InsP 7 and InsP 8 synthesis in vivo without
affecting levels of other inositol phosphates. TNP did not inhibit either human or yeast …
6) to yield 5-diphosphoinositol pentakisphosphate (5-[PP]-InsP 5 or InsP 7). In this study, we
report the characterization of a selective inhibitor, N 2-(m-(trifluoromethy) lbenzyl) N 6-(p-
nitrobenzyl) purine (TNP), for these enzymes. TNP dose-dependently and selectively
inhibited the activity of IP6K in vitro and inhibited InsP 7 and InsP 8 synthesis in vivo without
affecting levels of other inositol phosphates. TNP did not inhibit either human or yeast …