[HTML][HTML] A stable but reversible integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair
Y Wen, G Liao, T Pritchard, TT Zhao… - Journal of Biological …, 2017 - ASBMB
The discovery and application of CRISPR/Cas9 technology for genome editing has greatly
accelerated targeted mutagenesis in a variety of organisms. CRISPR/Cas9-mediated site-
specific cleavage is typically exploited for the generation of insertions or deletions (indels)
after aberrant dsDNA repair via the endogenous non-homology end-joining (NHEJ) pathway
or, alternatively, for enhancing homology-directed repair to facilitate the generation of a
specific mutation (or" knock-in"). However, there is a need for efficient cellular assays that …
accelerated targeted mutagenesis in a variety of organisms. CRISPR/Cas9-mediated site-
specific cleavage is typically exploited for the generation of insertions or deletions (indels)
after aberrant dsDNA repair via the endogenous non-homology end-joining (NHEJ) pathway
or, alternatively, for enhancing homology-directed repair to facilitate the generation of a
specific mutation (or" knock-in"). However, there is a need for efficient cellular assays that …
