SA-β-galactosidase-based screening assay for the identification of senotherapeutic drugs
H Fuhrmann-Stroissnigg, FE Santiago, D Grassi… - JoVE (Journal of …, 2019 - jove.com
Cell senescence is one of the hallmarks of aging known to negatively influence a healthy
lifespan. Drugs able to kill senescent cells specifically in cell culture, termed senolytics, can
reduce the senescent cell burden in vivo and extend healthspan. Multiple classes of
senolytics have been identified to date including HSP90 inhibitors, Bcl-2 family inhibitors,
piperlongumine, a FOXO4 inhibitory peptide and the combination of Dasatinib/Quercetin.
Detection of SA-β-Gal at an increased lysosomal pH is one of the best characterized …
lifespan. Drugs able to kill senescent cells specifically in cell culture, termed senolytics, can
reduce the senescent cell burden in vivo and extend healthspan. Multiple classes of
senolytics have been identified to date including HSP90 inhibitors, Bcl-2 family inhibitors,
piperlongumine, a FOXO4 inhibitory peptide and the combination of Dasatinib/Quercetin.
Detection of SA-β-Gal at an increased lysosomal pH is one of the best characterized …
