In humans, DNA methylation at specific CpG sites can be used to estimate the 'epigenetic
clock', a biomarker of aging and health. The mechanisms that regulate the aging epigenome
and level of conservation are not entirely clear. We performed affinity-based enrichment with
methyl-CpG binding domain protein followed by high-throughput sequencing (MBD-seq) to
assay DNA methylation in mouse samples. Consistent with previous reports, aging is
associated with increase in methylation at CpG islands that likely overlap regulatory regions …